nucleotide triphosphates ntps (New England Biolabs)

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New England Biolabs nucleotide triphosphates ntps
Nucleotide Triphosphates Ntps, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntps/T4+DNA+Ligase/pmc13076212-55-0-13
Average 99 stars, based on 33541 article reviews

nucleotide triphosphates ntps - by Bioz Stars, 2026-09

99/100 stars

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Purification:

Article Title: Rational design of synthetic proteins using a genome-scale CRISPR screen
Article Snippet: .. Next, reaction mixes containing 5 mM each of pseudouridine and NTPs (adenosine-, cytidine-, guanosine-5′-triphosphate, NEB E2040S), 4 mM mRNA Cap AG (Cap1), 2.5 μg of the purified template, 1 unit/μL murine RNase inhibitor (NEB M0314S), 0.002 units/μL inorganic yeast pyrophosphatase (NEB M2403S), and 50 units/μL T7 RNA polymerase (NEB M0251L) were assembled in 1x transcription buffer (100 μL total volume) and incubated for 3 hours at 37 °C. .. For in vitro transcription, we first made a 10 × transcription buffer: 400 mM Tris-HCL (IBI Scientific IB70162), 100 mM DTT (Sigma 43816), 20 mM spermidine (Sigma 85558), 0.02% Triton X-100 (Sigma 93443), and 165 mM magnesium acetate (Sigma 63052).

Article Title: Single-turnover kinetic analysis of non-long terminal repeat retrotransposition defines the pathway and rate constants leading to second-strand synthesis
Article Snippet: PCR was performed with Q5 polymerase (NEB) with 5% DMSO as an additive, then amplicons were purified with a PCR cleanup kit (Qiagen). .. In vitro transcription reactions were set up in a volume of 320 μl with 8 μg of purified PCR product, 5 mM DTT, 2 mM NTPs, and 1600 Units of T7 RNA polymerase in T7 RNA Polymerase reaction buffer (NEB). .. Reactions were incubated at 37°C for 3 h, then digested with 64 units of RNase-free DNase I (NEB) at 37°C for 15 min. EDTA was then added to 5 mM, and the reaction was heat-inactivated at 85°C for 10 min. RNA was then ethanol precipitated with Lithium Chloride by adding 8M Lithium Chloride to a final concentration of 0.8 M and ice-cold Ethanol to a final concentration of 75% (v/v).

Article Title: Single-turnover kinetic analysis of non-long terminal repeat retrotransposition defines the pathway and rate constants leading to second-strand synthesis.
Article Snippet: PCR was performed with Q5 polymerase (NEB) with 5% DMSO as an additive, then amplicons were purified with a PCR cleanup kit (Qiagen). .. In vitro transcription reactions were set up in a volume of 320 μl with 8 μg of purified PCR product, 5 mM DTT, 2 mM NTPs, and 1600 Units of T7 RNA polymerase in T7 RNA Polymerase reaction buffer (NEB). .. Reactions were incubated at 37 ◦C for 3 h, then digested with 64 units of RNase-free DNase I (NEB) at 37 ◦C for 15 min. EDTA was then added to 5 mM, and the reaction was heat-inactivated at 85 ◦C for 10 min. RNA was then ethanol precipitated with Lithium Chloride by adding 8M Lithium Chloride to a final concentration of 0.8 M and ice-cold Ethanol to a final concentration of 75% (v/v).

Incubation:

Concentration Assay:

Article Title: Demultiplexing and barcode-specific adaptive sampling for nanopore direct RNA sequencing
Article Snippet: .. DNA concentration was measured with Nanodrop and 250 fmol of DNA was then used in a 10 μL in vitro transcription reaction: 5 mM NTPs, 5 U/μL T7 RNA polymerase (homemade), 1 μL YIPP (NEB), 0.1 μL RNasin (Molox) in 40 mM Tris pH 7.5, 18 mM MgCl2, 10 mM DTT, 1 mM spermidine, 5 mM NTPs, 40 U RNasin (Molox) and homemade T7 RNA polymerase. .. After in vitro transcription for 2 h at 37 °C the DNA template was digested by addition of 10 μL of DNase mix containing 1 μL 10x DNase I buffer and 0.2 μL of DNase I (NEB) followed by a 15 min incubation at 37 °C.

In Vitro:

Article Title: Single-turnover kinetic analysis of non-long terminal repeat retrotransposition defines the pathway and rate constants leading to second-strand synthesis.
Article Snippet: PCR was performed with Q5 polymerase (NEB) with 5% DMSO as an additive, then amplicons were purified with a PCR cleanup kit (Qiagen). .. In vitro transcription reactions were set up in a volume of 320 μl with 8 μg of purified PCR product, 5 mM DTT, 2 mM NTPs, and 1600 Units of T7 RNA polymerase in T7 RNA Polymerase reaction buffer (NEB). .. Reactions were incubated at 37 ◦C for 3 h, then digested with 64 units of RNase-free DNase I (NEB) at 37 ◦C for 15 min. EDTA was then added to 5 mM, and the reaction was heat-inactivated at 85 ◦C for 10 min. RNA was then ethanol precipitated with Lithium Chloride by adding 8M Lithium Chloride to a final concentration of 0.8 M and ice-cold Ethanol to a final concentration of 75% (v/v).

Polymerase Chain Reaction:

Article Title: Single-turnover kinetic analysis of non-long terminal repeat retrotransposition defines the pathway and rate constants leading to second-strand synthesis.
Article Snippet: PCR was performed with Q5 polymerase (NEB) with 5% DMSO as an additive, then amplicons were purified with a PCR cleanup kit (Qiagen). .. In vitro transcription reactions were set up in a volume of 320 μl with 8 μg of purified PCR product, 5 mM DTT, 2 mM NTPs, and 1600 Units of T7 RNA polymerase in T7 RNA Polymerase reaction buffer (NEB). .. Reactions were incubated at 37 ◦C for 3 h, then digested with 64 units of RNase-free DNase I (NEB) at 37 ◦C for 15 min. EDTA was then added to 5 mM, and the reaction was heat-inactivated at 85 ◦C for 10 min. RNA was then ethanol precipitated with Lithium Chloride by adding 8M Lithium Chloride to a final concentration of 0.8 M and ice-cold Ethanol to a final concentration of 75% (v/v).

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